Journal: iScience

Article Title: Restored glyoxylate metabolism after AGXT gene correction and direct reprogramming of primary hyperoxaluria type 1 fibroblasts

doi: 10.1016/j.isci.2024.109530

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Article Snippet: Two defined culture media were employed sequentially, endoderm induction medium for 3-5 days (cDMEM supplemented with 4.99mM nicotinamide [Sigma, Cat#N0636], 10ng/ml recombinant human FGF-basic [Peprotech, Cat#100-18B], 20ng/ml recombinant human HGF [Peprotech, Cat#100-39], 0.10% DMSO [Sigma, Cat#D2438], 2μM A-83-01 [Sigma, Cat#SML0788] and 3μM CHIR99021 [Sigma, Cat#SML1046]) followed by hepatic maturation medium until day 13 (cDMEM supplemented with 0.1μM dexamethasone [Sigma, Cat#D8893], ITS (insulin, transferrin, sodium selenite) liquid media supplement 1X [Sigma, Cat#I3146], 640μM N-nitro-Larginine methyl ester hydrochloride [L-NAME; Sigma, Cat#N5751], 20ng/ml recombinant human HGF [Peprotech, Cat#100-39], 20ng/ml recombinant human oncostatin M [Peprotech, Cat#300-10], 0.10% DMSO [Sigma, Cat#D2438], 2μM A-83-01 [Sigma, Cat#SML0788] and 3μM CHIR99021 [Sigma, Cat#SML1046]).

Techniques: Recombinant, Virus, Derivative Assay, Cell Culture, PCR Cloning, SYBR Green Assay, Lactate Dehydrogenase Assay, Sequencing, Knock-In, Plasmid Preparation, Amplification, Expressing, Software, Extraction